WalK

• Description: two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors
  
  

Gene name	walK
Synonyms	yycG
Essential	yes PubMed
Product	two-component sensor kinase (OmpR family)
Function	control of cell wall metabolism
Gene expression levels in SubtiExpress: walK
Interactions involving this protein in SubtInteract: WalK
MW, pI	69 kDa, 4.775
Gene length, protein length	1833 bp, 611 aa
Immediate neighbours	yycH, walR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, protein modification, transcription factors and their control, essential genes, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU40400

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU40400

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of WalR

• Protein family: two-component sensor kinase of the OmpR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • WalK-WalR
  • WalK-YycH PubMed,
  • WalK-YycI PubMed
  • WalK-DivIB PubMed
  • WalK-FtsL PubMed
  • WalK-PbpB PubMed
  • WalK-FtsZ PubMed

• Localization:
  • cell membrane PubMed
  • part of the division septum in growing cells, interacts with the divisome PubMed

Database entries

• BsubCyc: BSU40400

• Structure:
  • 3SL2 (complex of the ATP binding histidine kinase domain with ATP) PubMed

• UniProt: Q45614

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-htrC PubMed

• Expression browser: walK PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 236 PubMed

Biological materials

• Mutant:

• Expression vector:
  • pGP2636, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

<pubmed>19019149,18792692,10094672, 18245295 </pubmed>

Original Publications

<pubmed>18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157, 19342776 20057515 20059685 20167622 21219466 23632918 22751669,22526318 24490131 24900578 </pubmed>