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| * '''Description:''' [[Protein kinases and phosphatases|protein phosphatase]] <br/><br/>
| | {{#bank: PrpC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''prpC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloO ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[Protein kinases and phosphatases|protein phosphatase]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || antagonist of [[PrkC]]-dependent phosphorylation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760 prpC]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrpC PrpC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=prpC prpC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 4.355
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 762 bp, 254 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloN]]'', ''[[prkC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15760 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:prpC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:prpC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15760]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15760
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| ===Phenotypes of a mutant ===
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| A ''prpC'' mutant is less lytic in late stationary phase. [http://www.ncbi.nlm.nih.gov/sites/entrez/12399479 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13390]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' A phosphoprotein + H<sub>2</sub>O = a protein + phosphate (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Proteins dephosphorylated by PrpC ===
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| [[CpgA]], [[tufA | EF-Tu]], [[YezB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [[ptsH | HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed], [[YkwC]] {{PubMed|24390483}}
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:''' divalent cations such as magnesium or manganese
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| * '''Effectors of protein activity:''' inhibited by inorganic phosphate and glycero-2-phosphate [http://www.ncbi.nlm.nih.gov/sites/entrez/16857667 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[YvcK]]-[[PrpC]] {{PubMed|25012659}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15760&redirect=T BSU15760]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1TXO 1TXO] (from ''Mycobacterium tuberculosis'', 34% identity, 54% similarity) {{PubMed|15530359}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34779 O34779]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15760]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.16 3.1.3.16]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[prpC]]-[[prkC]]-[[cpgA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/16025310?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prpC_1650384_1651148_1 prpC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 459 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' OMG401 (aphA3), available in the [[Stülke]] lab
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| ** 1A962 (no resistance), {{PubMed|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A962&Search=1A962 BGSC]
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| ** 1A964 (no resistance), {{PubMed|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 21372323 </pubmed>
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| ==Original publications==
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| <pubmed>19246764,10986276,17693724 , 12399479, 18757537, 15530359 23793375 16025310 24390483 25012659</pubmed>
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| [[Category:Protein-coding genes]]
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