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| * '''Description:''' [[two-component systems|two-component]] response regulator ([[transcription factors of the OmpR family|OmpR family]]), regulation of phosphate metabolism <br/><br/>
| | {{#bank: PhoP}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''phoP''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator ([[transcription factors of the OmpR family|OmpR family]])
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of [[phosphate metabolism]] <br/>(''[[phoA]], [[phoB]], [[phoD]], [[resA | resABCDE]], [[tagA]]-[[tagB]], [[tagD | tagDEF]], [[tuaA | tuaA-H]]'')
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29110 phoP]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PhoP PhoP]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=phoP phoP]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.068
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 720 bp, 240 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phoR]]'', ''[[mdh]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29110 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29110 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29110 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:phoP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phoP_2977800_2978522_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:phoP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29110]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphate metabolism]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[regulators of core metabolism]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[PhoP regulon]]}},
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| {{SubtiWiki regulon|[[SigB regulon]]}},
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| {{SubtiWiki regulon|[[SigE regulon]]}}
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| =The [[PhoP regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU29110
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29110&redirect=T BSU29110]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoPR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10363]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[transcription factors of the OmpR family|OmpR family]] of two-component response regulators
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation by [[PhoR]] under conditions of phosphate limitation (stimulates DNA-binding activity)
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' phosphorylation stimulates DNA-binding activity
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| * '''[[SubtInteract|Interactions]]:''' [[PhoP]]-[[PhoP]], [[PhoP]]-[[PhoR]]
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29110&redirect=T BSU29110]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MVO 1MVO] (receiver domain)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P13792 P13792]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29110]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[phoP]]-[[phoR]]'' {{PubMed|14762014}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phoP_2977800_2978522_-1 phoP] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|16452408}}, [[SigB]] {{PubMed|15205429}}, [[SigE]] {{PubMed|15699190,15205429}}
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| * '''Regulation:'''
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| ** carbon catabolite repression ([[CcpA]]) {{PubMed|16452408,12850135}}
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| ** expressed under conditions of phosphate limitation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15205429,14762014 PubMed]
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| ** expressed in post-exponential phase ([[ScoC]]) {{PubMed|20382764}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription repression {{PubMed|16452408,12850135}}
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| ** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/15205429,14762014 PubMed]
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| ** [[ScoC]]: transcription repression {{PubMed|20382764}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 90 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 706 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A966 ( ''phoP''::''tet''), {{PubMed|14973033}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Marion Hulett]], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
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| =Your additional remarks=
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| =References=
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| ==Regulation of ''[[phoP]]-[[phoR]]'' expression==
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| <pubmed>20382764 14762014,12850135,15205429,16452408,</pubmed>
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| ==Biochemical analyses==
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| <pubmed>9680208, 9611818,12486063, 20167622 10433720, 17085571,14973033,12486062,</pubmed>
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| ==Targets of PhoR==
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| '''Additional publications:''' {{PubMed|21636651}}
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| <pubmed>20059685 16030210, 10913081,10094677,9683503,9988472,9457886,9335276,9593301,9098050,</pubmed>
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| ==Other original publications==
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| <pubmed>10094672, 15576792,</pubmed>
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| [[Category:Protein-coding genes]]
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