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| * '''Description:''' [[Protein kinases and phosphatases|protein serine phosphatase]], septum-associated PP2C, dephosphorylation of [[SpoIIAA]] <br/><br/>
| | {{#bank: SpoIIE}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spoIIE''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIH, spoIIK ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[Protein kinases and phosphatases|protein serine phosphatase]], septum-associated PP2C
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigF]] activity <br/>required for normal formation of the asymmetric septum
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00640 spoIIE]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SpoIIE SpoIIE]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=spoIIE spoIIE]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 91 kDa, 6.137
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2481 bp, 827 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trnSL-Glu1]]'', ''[[yabS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00640 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00640 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00640 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spoIIE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIE_70538_73021_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spoIIE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00640]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[sigma factors and their control]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU00640
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00640&redirect=T BSU00640]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10127]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' dephosphorylation of [[SpoIIAA]]
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| * '''Protein family:''' PP2C [[Protein kinases and phosphatases|phosphatase]]
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SpoIIE]]-[[FtsZ]]
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| ** [[SpoIIE]]-[[SpoIIAB]]
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| ** [[SpoIIE]]-[[SpoIIE]]
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| ** [[SpoIIQ]]-[[SpoIIE]]
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| ** forms a dimer {{PubMed|22115775}}
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| * '''[[Localization]]:'''
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| ** cell membrane (according to Swiss-Prot)
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| ** localizes to the polar cell division sites where it causes [[FtsZ]] to relocate from mid-cell to form polar Z-rings
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00640&redirect=T BSU00640]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=3T91 3T91] (phosphatase domain) {{PubMed|22115775}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37475 P37475]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00640]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.16 3.1.3.16]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''spoIIE'' (DBTBS)
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIE_70538_73021_1 spoIIE] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084 PubMed]
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| * '''Regulation:'''
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| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
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| =Your additional remarks=
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| =References=
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| ==Modeling of [[SigF]] activation==
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| <pubmed> 24067622 22312331</pubmed>
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| ==Original Publications==
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| <pubmed>7570023,7570022,12180929,9077448,9364910,10323866,8622920,12007411,16824103,10476035,10747015,10322028,15978076,9495766,14744853,9642177,15866939,8846916,1556084,1948031,9573053,18077456,11846550,9150232,15205443,1556084,15687200, 19578359 10049355 20817757 22115775</pubmed>
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| [[Category:Protein-coding genes]]
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