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YcsF: Difference between revisions

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* '''Description:''' similar to lactam utilization protein <br/><br/>
{{#bank: YcsF}}
{| align="right" border="1" cellpadding="2"
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ycsF''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04050 ycsF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ycsF ycsF]'''
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.582 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 771 bp, 257 aa
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycsE]]'', ''[[ycsG]]''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04050 DNA_with_flanks]
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:ycsF_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycsF_457023_457796_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ycsF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04050]]
|-
|}


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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[poorly characterized/ putative enzymes]]}}
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[KipR regulon]]}},
{{SubtiWiki regulon|[[TnrA regulon]]}}
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU04050
===Phenotypes of a mutant ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04050&redirect=T BSU04050]
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11227]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:'''
* '''Protein family:''' UPF0271 (lamB) family (according to Swiss-Prot)
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:'''
* '''[[Localization]]:'''
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04050&redirect=T BSU04050]
* '''Structure:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42963 P42963]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04050]
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:''' ''[[ycsF]]-[[ycsG]]-[[ycsI]]-[[kipI]]-[[kipA]]-[[kipR]]-[[lipC]]'' {{PubMed|9334321}}
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ycsF_457023_457796_1 ycsF] {{PubMed|22383849}}
* '''Sigma factor:'''
* '''Regulation:'''
* '''Regulatory mechanism:'''
** [[KipR]]: transcription repression {{PubMed|9334321}}
** [[TnrA]]: transcription activation {{PubMed|9334321}}
* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 382 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 181 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 371 {{PubMed|21395229}}
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
<pubmed>9334321 </pubmed>
[[Category:Protein-coding genes]]

Latest revision as of 08:34, 30 July 2015