|
|
| Line 1: |
Line 1: |
| * '''Description:''' heme monooxygenase<br/><br/>
| | {{#bank: HmoB}} |
| {| align="right" border="1" cellpadding="2" | |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''hmoB''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yixC, yhgC ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || heme monooxygenase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || degradation of heme, [[acquisition of iron]]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10100 hmoB]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=HmoB HmoB]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.216
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 498 bp, 166 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhgB]]'', ''[[pbpF]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10100 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:yhgC_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yhgC_1083229_1083729_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hmoB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10100]]
| |
| |-
| |
| |}
| |
|
| |
|
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[acquisition of iron]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
|
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU10100
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10100&redirect=T BSU10100]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yhgC.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10427]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor {{PubMed|21873409}}
| |
|
| |
| * '''Protein family:'''
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10100&redirect=T BSU10100]
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3TVZ 3TVZ] {{PubMed|22531134}}
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P38049 P38049]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10100]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[hmoB]]'' {{PubMed|8335642}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yhgC_1083229_1083729_-1 hmoB] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
|
| |
| * '''Regulation:'''
| |
| ** constitutively expressed {{PubMed|21873409}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 727 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1160 {{PubMed|24696501}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| <pubmed> 8335642 22531134 24582752 21873409</pubmed>
| |
| [[Category:Protein-coding genes]]
| |
