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| * '''Description:''' [[cell shape]]-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for [[CwlO]] activity <br/><br/>
| | {{#bank: Mbl}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mbl''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[MreB]]-like protein
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[cell shape]] determination
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410 mbl]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Mbl Mbl]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.669
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhO]]'', ''[[spoIIID]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36410 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36410 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36410 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mbl_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mbl_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36410]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell shape]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigE regulon]]}},
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| {{SubtiWiki regulon|[[stringent response]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36410
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| ===Phenotypes of a mutant ===
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| * non-viable in the presence of low Mg(2+)
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| * readily accumulate ''[[rsgI]]'' suppressor mutants [http://www.ncbi.nlm.nih.gov/sites/entrez/19114499 PubMed]
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36410&redirect=T BSU36410]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/usd-spoIIID-mbl-flhOP.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10916]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** forms helical filaments in a heterologous system {{PubMed|21091501}}
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| ** required for [[CwlO]] activity {{PubMed|23869552}}
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| * '''Protein family:''' ftsA/mreB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[MreB]], [[MreBH]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** part of the [[cell wall biosynthetic complex]] {{PubMed|21636744,21636745}}
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| ** [[MreB]]-[[Mbl]] {{PubMed|21636744}}
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| ** [[Mbl]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
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| ** [[RodZ]]-[[Mbl]] {{PubMed|23879732}}
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| * '''[[Localization]]:'''
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| ** during logarithmic growth, [[Mbl]] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis {{PubMed|21636744}}
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| ** forms transverse bands as cells enter the stationary phase {{PubMed|21636744}}
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| ** close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]
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| ** reports on helical structures formed by Mbl {{PubMed|16950129,20566861}} seem to be misinterpretation of data {{PubMed|21636744}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36410&redirect=T BSU36410]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39751 P39751]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36410]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[usd]]-[[spoIIID]]-[[mbl]]-[[flhO]]-[[flhP]]'' {{PubMed|9023218,7836311}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mbl_3747254_3748255_-1 mbl] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9023218}}
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| * '''Regulation:'''
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| ** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|9023218}}
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| ** expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) {{PubMed|22048160}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** An [[ncRNA|antisense RNA]] is predicted for ''[[mbl]]'' {{PubMed|20525796}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 336 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2526 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
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| * '''Antibody:''' available in the [[Jeff Errington]] and [[Peter Graumann]] labs
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| =Labs working on this gene/protein=
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| [[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
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| =Your additional remarks=
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| =References=
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| ==Localization==
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| <pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed>
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| ==Other original publications==
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| <pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed>
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| [[Category:Protein-coding genes]]
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