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| * '''Description:''' [[trigger enzyme]],hypoxanthine phosphoribosyltransferase and part of a transcription activator<br/><br/>
| | {{#bank: HprT}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''hprT''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || hypoxanthine phosphoribosyltransferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion, control of ''[[ftsH]]'' expression
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00680 hprT]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=HprT HprT]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=hprT hprT]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 4.603
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 540 bp, 180 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tilS]]'', ''[[ftsH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00680 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:hprT_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hprT_76344_76886_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hprT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00680]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigM regulon]]}}
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| = The [[TilS-HprT regulon]] =
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| * ''[[ftsH]]''
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU00680
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00680&redirect=T BSU00680]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10131]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
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| * '''Protein family:''' purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´{{PubMed|22981860}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[TilS]]-[[HprT]], the complex activates transcription of ''[[ftsH]]'' {{PubMed|24001521}}
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00680&redirect=T BSU00680]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1J7J 1J7J] (from ''Salmonella typhimurium'', 51% identity, 73% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37472 P37472]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00680]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.4.2.8 2.4.2.8]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[tilS]]-[[hprT]]-[[ftsH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hprT_76344_76886_1 hprT] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1240 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 5379 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 18179421 22981860 24001521 </pubmed>
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| [[Category:Protein-coding genes]]
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