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| * '''Description:''' ribose-5-phosphate isomerase <br/><br/>
| | {{#bank: YwlF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ywlF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipc-32d ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ribose-5-phosphate isomerase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36920 ywlF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ywlF ywlF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.416
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlG]]'', ''[[ywlE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36920 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywlF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlF_3791199_3791648_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ywlF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36920]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[TnrA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36920
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36920&redirect=T BSU36920]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywlFG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10942]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' lacAB/rpiB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Reversible Mass Action Kinetics [http://www.ncbi.nlm.nih.gov/sites/entrez/956158 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** Inhibited by 6-phosphogluconate and AMP [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| ** Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ [http://www.ncbi.nlm.nih.gov/sites/entrez/956158 PubMed]
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| ** Activated by EDTA and 2-mercaptoethanol [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36920&redirect=T BSU36920]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3HEE 3HEE] (from ''Clostridium thermocellum'', 55% identity, 72% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39156 P39156]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36920]
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| * '''E.C. number:''' [http://www.expasy.ch/cgi-bin/nicezyme.pl?5.3.1.6 5.3.1.6]
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| === Additional information===
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| The enzyme has probably more than one active site, but with no cooperativity described [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| =Expression and regulation=
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| * '''Operon:''' ''[[ywlF]]-[[ywlG]]'' {{PubMed|12823818}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlF_3791199_3791648_-1 ywlF] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}}
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| * '''Regulatory mechanism:'''
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| ** [[TnrA]]: transcription repression {{PubMed|12823818}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 367 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1467 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| * '''FLAG-tag construct:''' GP1404 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12823818, 19446032,12823818, 19446032 956158 7144591 956158</pubmed>
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| [[Category:Protein-coding genes]]
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