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| * '''Description:''' ribonucleoside-diphosphate reductase (major subunit) <br/><br/>
| | {{#bank: NrdF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''nrdF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ribonucleoside-diphosphate reductase (major subunit)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17390 nrdF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=NrdF NrdF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=nrdF nrdF]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 4.66
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17390 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nrdF_1871089_1872078_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:nrdF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17390]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ResD regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17390
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17390&redirect=T BSU17390]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nrdIEF-ymaB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11405]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H<sub>2</sub>O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
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| * '''Protein family:''' ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[YosP]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| ** Mn(2+) {{PubMed|21561096}}
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| ** dimanganic-tyrosyl radical (Mn<sup>III</sup><sub>2</sub>-Y•) cofactor {{PubMed|24401092}}
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[NrdE]]-[[NrdF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| ** [[NrdI]]-[[NrdF]] {{PubMed|23402532}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17390&redirect=T BSU17390]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4DR0 4DR0] {{PubMed|22443445}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P50621 P50621]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17390]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' {{PubMed|16885274}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nrdF_1871089_1872078_1 nrdF] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|16885274}}
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| * '''Regulation:'''
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| ** expressed under anaerobic conditions ([[ResD]]) {{PubMed|16885274}}
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| * '''Regulatory mechanism:'''
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| ** [[ResD]]: transcription activation {{PubMed|16885274}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 288 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 864 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Elisabeth Härtig]], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 17229208 </pubmed>
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| ==Original Publications==
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| <pubmed>16885274,8969495 24401092 23402532,22443445,21561096</pubmed>
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| [[Category:Protein-coding genes]]
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