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| * '''Description:''' GTP-binding protein, putative tRNA modification GTPase<br/><br/>
| | {{#bank: ThdF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''thdF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''mnmE ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || putative tRNA modification GTPase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU41020 thdF]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ThdF ThdF]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 4.643
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1377 bp, 459 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gidA]]'', ''[[jag]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU41020 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU41020 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU41020 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ThdF_jag_spoIIIJ_rnpA_rpmH_context.png]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trmE_4211510_4212889_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:thdF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU41020]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[GTP-binding proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ComK regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU41020
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU41020&redirect=T BSU41020]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/thdF-gidAB-yyaA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10060]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
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| * '''Protein family:''' MnmE subfamily (according to Swiss-Prot) Era/Obg family
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| * '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[GidA]]-[[ThdF]] (suggested by interaction of ''E. coli'' counterparts {{PubMed|19801413}} and by co-localization on the chromosome)
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU41020&redirect=T BSU41020]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P25811 P25811]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU41020]
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| * '''E.C. number:'''
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| === Additional information===
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| Information on the corresponding protein from ''E. coli'': [http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10997-MONOMER EcoCyc]
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| MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with [[GidA]]. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.
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| =Expression and regulation=
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| * '''Operon:''' ''[[thdF]]-[[gidA]]-[[rsmG]]-[[noc]]'' {{PubMed|11807071}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trmE_4211510_4212889_-1 thdF] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[ComK]]: transcription activation {{PubMed|11948146}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 180 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 507 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Naotake Ogasawara]], Nara, Japan
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 21885683</pubmed>
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| ==Original publications==
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| <pubmed>12427945, 11807071 11948146 23630314 </pubmed>
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| [[Category:Protein-coding genes]]
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