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| * '''Description:''' GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit <br/><br/>
| | {{#bank: Obg}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''obg''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || Yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || GTP-binding protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly, may be required to stimulate activity <br/>
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| of the [[phosphorelay]] that activates [[Spo0A]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27920 obg]
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| of the [[phosphorelay]] that activates [[Spo0A]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Obg Obg]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.859
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[pheB]]'', ''[[spo0B]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27920 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:obg_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=obg_2852661_2853947_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:obg_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27920]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[essential genes]]}},
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| {{SubtiWiki category|[[GTP-binding proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU27920
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27920&redirect=T BSU27920]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0B-obg.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10337]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
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| * '''Protein family:''' GTP1/OBG family (according to Swiss-Prot) Era/Obg family
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| * '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| GDP, GTP and probably ppGpp
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[RplM]]-[[Obg]] {{PubMed|10781545,20830302}}
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| Interaction with Rsb proteins.
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27920&redirect=T BSU27920]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1LMZ 1LMZ], [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1LNZ 1LNZ]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P20964 P20964]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27920]
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| * '''E.C. number:'''
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| === Additional information===
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| The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.
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| =Expression and regulation=
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| * '''Operon:''' ''[[spo0B]]-[[obg]]'' {{PubMed|2537815}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=obg_2852661_2853947_-1 obg] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|2537815}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 406 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 981 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP835, available in [[Stülke]] lab
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| ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP843, available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| *[[Bill Haldenwang]], San Antonio, USA
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| *[[Naotake Ogasawara]], Nara, Japan
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>19575570 21885683</pubmed>
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| ==Original publications==
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| <pubmed>15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966, 12427945,12429099 2537815 20830302</pubmed>
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| [[Category:Protein-coding genes]]
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