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| * '''Description:''' transcriptional repressor of gluconeogenetic genes and of [[sr1]]. repression in the presence of glucose <br/><br/>
| | {{#bank: CcpN}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ccpN''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqzB ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || repressor of genes involved in <br/>gluconeogenesis (''[[gapB]]'','' [[pckA]]'') and of ''[[sr1]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25250 ccpN]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CcpN CcpN]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ccpN ccpN]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23.4 kDa, 7.22
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 636 bp, 212 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfL]]'', ''[[glyS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25250 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yqzB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ccpN_2604959_2605597_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ccpN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25250]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[regulators of core metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The [[CcpN regulon]]:=
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| * ''[[gapB]]-[[speD]]'', ''[[pckA]]'', ''[[Sr1]]''
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU25250
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| ===Phenotypes of a mutant ===
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| Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway [http://www.ncbi.nlm.nih.gov/sites/entrez/18586936 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25250&redirect=T BSU25250]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ccpN-yqfL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13768]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' transcription repression of the ''[[gapB]]'', ''[[pckA]]'', and ''[[sr1]]'' genes in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** [[YqfL]] (negative effector of CcpN activity) [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| ** ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN [http://www.ncbi.nlm.nih.gov/sites/entrez/18511073 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| ** active as dimer (according to {{PubMed|20408793}})
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| ** [[CcpN]]-[[RpoA]] {{PubMed|19726675}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25250&redirect=T BSU25250]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34994 O34994]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25250]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ccpN]]''-''[[yqfL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ccpN_2604959_2605597_-1 ccpN] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' constitutively expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| * '''Additional information:'''
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| ** the intracellular concentration of CcpN is about 4 myM (according to {{PubMed|20408793}}).
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 111 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 220 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| **DB104 ''ccpN''::cat, available in [[Sabine Brantl]]'s lab
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| **GP1128 ''ccpN''::cat, available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''Antibody:''' available in [[Sabine Brantl]]'s lab
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| =Labs working on this gene/protein=
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| [[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
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| [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]
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| [[Uwe Sauer]], ETH Zürich, Switzerland [http://www.imsb.ethz.ch/researchgroup/sauer homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20408793 </pubmed>
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| ==Original Publications==
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| <pubmed>15720552 16164558 17011578 18511073 18586936, 19726675 19732150 22190493 23280112 </pubmed>
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| [[Category:Protein-coding genes]]
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