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| * '''Description:''' transcriptional repressor of the SOS regulon <br/><br/>
| | {{#bank: LexA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''lexA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''dinR ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor of the SOS regulon
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of DNA damage repair
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17850 lexA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=lexA lexA]'''
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=LexA LexA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.155
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 615 bp, 205 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fosB]]'', ''[[yneA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17850 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:lexA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lexA_1917639_1918256_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:lexA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17850]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[LexA regulon]]}}
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| =The [[LexA regulon]]=
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17850
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17850&redirect=T BSU17850]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lexA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10678]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Represses ''[[uvrB]], [[dinB]], [[dinC]], [[recA]]'' genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, [[RecA]] interacts with [[LexA]] causing an autocatalytic cleavage which disrupts the DNA-binding part of [[LexA]], leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
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| * '''Protein family:''' peptidase S24 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17850&redirect=T BSU17850]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1JHH 1JHH] (from ''E. coli'', S119A mutant, 33% identity, 52% similarity) {{PubMed|11551506}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P31080 P31080]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17850]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[lexA]]'' {{PubMed|1657879}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lexA_1917639_1918256_-1 lexA] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** induced upon DNA damage ([[LexA]]) {{PubMed|1657879,8899710,16267290}}
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| * '''Regulatory mechanism:''' autorepression
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| ** [[LexA]]: transcription repression {{PubMed|1657879,8899710,16267290}}
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| * '''Additional information:'''
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| ** A [[ncRNA]] is predicted between ''[[fosB]]'' and ''[[lexA]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 22933559 18726173 </pubmed>
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| == Original publications ==
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| <pubmed>8899710, 9045831 9555905 8969214, 1657879,16267290 11551506 16549676 20525796 </pubmed>
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| [[Category:Protein-coding genes]]
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