SpsL

• Description: dTDP -4-dehydrorhamnose-3,5-epimerase, spore coat polysaccharide synthesis
  
  

Gene name	spsL
Synonyms	ipa-73d
Essential	no
Product	dTDP -4-dehydrorhamnose-3,5-epimerase
Function	rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsL
MW, pI	17 kDa, 4.878
Gene length, protein length	453 bp, 151 aa
Immediate neighbours	sivA, spsK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU37810

Database entries

• BsubCyc: BSU37810

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37810

• Structure: 3RYK (dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Bacillus anthracis str. Ames with TDP and PPi bound, 32% identity, 56% similarity)

• UniProt: Q7WY56

• KEGG entry: [3]

• E.C. number: 5.1.3.13

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsL PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>9353933 15383836 22960854 25239894</pubmed>