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| * '''Description:''' UDP-N-acetylglucosamine 1-carboxyvinyltransferase <br/><br/>
| | {{#bank: MurAB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''murAB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''murZ, lssF, rev-4 ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || UDP-N-acetylglucosamine 1-carboxyvinyltransferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37100 murAB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=murAB murAB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 5.831
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1287 bp, 429 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glpX]]'', ''[[ywjH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37100 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:murAB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAB_3806086_3807375_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:murAB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37100]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall synthesis]]}},
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| {{SubtiWiki category|[[biosynthesis of cell wall components]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37100
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37100&redirect=T BSU37100]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10414]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
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| * '''Protein family:''' MurA subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[MurAA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37100&redirect=T BSU37100]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3SG1 3SG1] (from ''B. anthracis'', 50% identity, 81% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P19670 P19670]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37100]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.5.1.7 2.5.1.7]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''''' [[murAB]]-[[glpX]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19270101 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=murAB_3806086_3807375_-1 murAB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/19270101 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| **pGP2595: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 21822642 </pubmed>
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| == Original publications ==
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| <pubmed>19270101 </pubmed>
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| [[Category:Protein-coding genes]]
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