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| * '''Description:''' phosphate [[ABC transporter]] (ATP-binding protein) <br/><br/>
| | {{#bank: PstBA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pstBA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pstB, yqgJ, yzmE ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphate [[ABC transporter]] (ATP-binding protein)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || high-affinity phosphate uptake
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24960 pstBA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PstBA PstBA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 7.897
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pstBB]]'', ''[[pstA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24960 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pstBA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pstBA_2578003_2578812_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pstBA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24960]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ABC transporters]]}},
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| {{SubtiWiki category|[[phosphate metabolism]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PhoP regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24960
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24960&redirect=T BSU24960]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11378]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + H<sub>2</sub>O + phosphate(Out) = ADP + phosphate + phosphate(In) (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** ([[PstBA]]-[[PstBB]])-([[PstA]]-[[PstC]])-[[PstS]] {{PubMed|10092453}}
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24960&redirect=T BSU24960]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P46341 P46341]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24960]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.6.3.27 3.6.3.27]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[pstS]]-[[pstC]]-[[pstA]]-[[pstBA]]-[[pstBB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pstBA_2578003_2578812_-1 pstBA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
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| * '''Regulation:'''
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| ** expressed under conditions of phosphate limitation (5000-fold induced) ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[PhoP]]-P: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
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| * '''Additional information:'''
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| ** mRNA processing between ''[[pstS]]'' and ''[[pstC]]'' to maintain higher concentrations of [[PstS]] relative to other components of the [[ABC transporter]] {{PubMed|15289558}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Marion Hulett]], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>15289558,10092453,12897025,10913081,9098050,9098050,9593301, </pubmed>
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| [[Category:Protein-coding genes]]
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