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| * '''Description:''' [[trigger enzymes|trigger enzyme]]: major glucose permease of the [[PTS]], EIICBA(Glc) and control of [[GlcT]] activity <br/><br/>
| | {{#bank: PtsG}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ptsG''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ptsX, crr''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: glucose-specific enzyme IICBA component of the [[PTS]]
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| |style="background:#ABCDEF;" align="center"|'''Function''' || glucose transport and phosphorylation, control of [[GlcT]] activity
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890 ptsG]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsG PtsG]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ptsG ptsG]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 75,3 kDa, 5.40
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2097 bp, 699 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glcT]]'', ''[[ptsH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13890 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ptsG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsG_1457187_1459286_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ptsG_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphotransferase systems]]}},
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[trigger enzyme]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GlcT regulon]]}},
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| {{SubtiWiki regulon|[[stringent response]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13890
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13890&redirect=T BSU13890]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10198]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' transport and phosphorylation of glucose, receives a phosphate from [[PtsH |HPr]] at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator [[GlcT]].
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| * '''Protein family:''' [[PTS]] permease, glucose permease (Glc) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed], [[PTS]] enzyme II
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** 11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
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| ** PTS EIIC domain ( 1-424)
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| ** PTS EIIB domain (439–520)
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| ** PTS EIIA domain (568–672)
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| * '''Modification:''' transient phosphorylation ([[PtsH|HPr]]-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[PtsH|HPr]]-[[PtsG]] {{PubMed|8418852}}
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| ** PtsG-[[GlcT]] (for phosphorylation of [[GlcT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14527945 PubMed]
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| * '''[[Localization]]:''' membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13890&redirect=T BSU13890]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1AX3 1AX3] (IIA domain), [http://www.rcsb.org/pdb/explore.do?structureId=1GPR 1GPR] (IIA domain), IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=56258 NCBI], NMR IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=55395 NCBI]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P20166 P20166]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13890]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsG_1457187_1459286_1 ptsG] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11902727}}
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| * '''Regulation:'''
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| ** expression activated by glucose (32 fold) ([[GlcT]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| ** subject to negative stringent control upon lysine starvation {{PubMed|20081037}}
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| * '''Regulatory mechanism:'''
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| ** transcriptional antitermination via the [[GlcT]]-dependent [[RNA switch]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+9765562 PubMed]
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| ** stringent response: due to presence of guanine at +1 position of the transcript {{PubMed|20081037}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutants:''' all available in [[Jörg Stülke]]'s lab
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| ** QB5435 (cat) {{PubMed|11902727}}
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| ** QB5436 (spc)
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| ** QB5445 (erm)
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| ** GP777 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette), {{PubMed|22722928}}
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| ** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette), {{PubMed|22722928}}
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| * '''Expression vector:'''
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| ** pGP123 (domains BA, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| ** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| ** pGP428 (EIIB, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
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| ** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| ** pGP34 ([[pAC5]]) {{PubMed|11902727}}, available in [[Jörg Stülke]]'s lab
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| ** pGP66 ([[pAC7]]) {{PubMed|11902727}}, available in [[Jörg Stülke]]'s lab
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| ** pGP606 (mutant terminator, [[pAC6]]), available in [[Jörg Stülke]]'s lab
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| ** pGP532 ([[pAC7]]), available in [[ Jörg Stülke]]'s lab
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| ** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[Jörg Stülke]]'s lab
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| ** series of RAT mutants are available in [[pAC6]], available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany
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| [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 18086213 </pubmed>
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| ==Original publications==
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| <pubmed>10627040 ,12850135 ,18763711 ,11902727 ,9765562 ,9513271 ,1956301 ,10543968 ,17074746 ,15155854 ,14527945 ,1508157 ,2120236 9593197 8418852 1581296 1316146 1733770 1942043 1911744 1906345 20081037 22846916 </pubmed>
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| [[Category:Protein-coding genes]]
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