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| * '''Description:''' glucosamine-6-phosphate deaminase <br/><br/>
| | {{#bank: GamA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gamA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybfT ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glucosamine-6-phosphate deaminase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || glucosamine utilization
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02360 gamA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gamA gamA]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.793
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 747 bp, 249 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gamP]]'', ''[[gamR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02360 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gamA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nagBB_256823_257572_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gamA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02360]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell wall degradation/ turnover]]}},
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GamR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU02360
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| ===Phenotypes of a mutant ===
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| * no growth on glucosamine {{PubMed|23667565}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU02360&redirect=T BSU02360]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12746]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' d-glucosamine 6-phosphate + H<sub>2</sub>O = D-fructose 6-phosphate + NH<sub>3</sub> (according to Swiss-Prot)
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| * '''Protein family:''' NagB subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[NagB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:''' K(M): 3.0 mM {{PubMed|14343123}}
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU02360&redirect=T BSU02360]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2bkx 2BKX] (the structure of [[NagB]], 50% identity) {{PubMed|15755726}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31458 O31458]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02360]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[gamA]]-[[gamP]]'' {{PubMed|23667565}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nagBB_256823_257572_-1 gamA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|23667565}}
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| * '''Regulation:'''
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| ** induced by N-acetylglucosamine {{PubMed|14343123}}
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| ** induced by glucosamine ([[GamR]]) {{PubMed|24673833,23667565}}
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| * '''Regulatory mechanism:'''
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| ** [[GamR]]: transcription repression {{PubMed|24673833,23667565}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>10627040, 14343123 23667565 23876412 24673833,15755726 </pubmed>
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| [[Category:Protein-coding genes]]
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