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| * '''Description:''' large conductance [[transport proteins|mechanosensitive channel]] protein, prevents selective release of cytoplasmic proteins in a hypotonic environment <br/><br/>
| | {{#bank: MscL}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mscL''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywpC ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || large conductance [[transport proteins|mechanosensitive channel]] protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || resistance to osmotic downshockglycine betaine export
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360 mscL]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mscL mscL]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.649
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 390 bp, 130 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywpD]]'', ''[[ywpB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36360 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mscL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mscL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36360]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[coping with hypo-osmotic stress]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36360
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| ===Phenotypes of a mutant ===
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| sensitive to osmotic downshock (> 0.5 M) during logarithmic growth [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mscL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12497]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' mscL family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3HZQ 3HZQ] (MscL of ''Staphylococcus aureus'') {{PubMed|19701184}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94585 P94585]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36360]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''mscL'' {{PubMed|9353933}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mscL_3743267_3743659_-1 mscL] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:''' expressed in logarithmic phase [http://www.ncbi.nlm.nih.gov/sites/entrez/19252899 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A957 ( ''mscL''::''spec''), {{PubMed|18310427}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A957&Search=1A957 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Jan Maarten van Dijl]], Groningen, Netherlands
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| [[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>12626684 ,19727188 , 20825352 22685280 24258154 </pubmed>
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| ==Original Publications==
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| <pubmed>17665170,18310427,19160392,16897034,19252899, 19701184 9632260 19738010 9353933 </pubmed>
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| [[Category:Protein-coding genes]]
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