Revision as of 12:58, 2 April 2014

Description: surfactin synthetase / competence

Gene name	srfAD
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
*Gene expression levels in SubtiExpress*: srfAD
MW, pI	27 kDa, 5.22
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	srfAC, ycxA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, most abundant proteins

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

Locus tag: BSU03520

Phenotypes of a mutant

Database entries

BsubCyc: BSU03520

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: thioesterase family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactors:

Effectors of protein activity:

Interactions:

Localization: cytoplasm (according to Swiss-Prot)

Database entries

BsubCyc: BSU03520

Structure: 2RON, 2K2Q

UniProt: Q08788

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

Expression browser: srfAD PubMed

Sigma factor: SigA PubMed

Regulation:
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed
- repressed during hydrogen peroxide stress (PerR) PubMed
- expressed at high cell density (ComA) PubMed

Regulatory mechanism:
- CodY: transcription repression PubMed
- ComA: transcription activation PubMed
- PerR: transcription activation PubMed
- Spx: transcription repression PubMed
- Abh: transcription repressionPubMed

Additional information:
- belongs to the 100 most abundant proteins PubMed

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

@@ Line 58: / Line 58: @@
 === Database entries ===
+* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03520&redirect=T BSU03520]
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html]
@@ Line 92: / Line 93: @@
 === Database entries ===
+* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03520&redirect=T BSU03520]
 * '''Structure:''' [http://www.proteopedia.org/wiki/index.php/2ron 2RON], [http://www.proteopedia.org/wiki/index.php/2k2q 2K2Q]