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| * '''Description:''' extracellular neutral protease B <br/><br/>
| | {{#bank: NprE}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''nprE''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || extracellular neutral protease B
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| |style="background:#ABCDEF;" align="center"|'''Function''' || degradation of proteins
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14700 nprE]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 7.481
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1563 bp, 521 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yktD]]'', ''[[ylaA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14700 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:nprE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nprE_1540036_1541601_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:nprE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14700]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}},
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| {{SubtiWiki category|[[proteolysis]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[ScoC regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14700
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nprE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10448]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Similar, but not identical, to that of thermolysin (according to Swiss-Prot)
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| * '''Protein family:''' peptidase M4 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[NprB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1NPC 1NPC] (of ''Bacillus cereus'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P68736 P68736]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14700]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''nprE'' (according to [http://dbtbs.hgc.jp/COG/prom/nprE.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nprE_1540036_1541601_-1 nprE] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** repressed during exponential growth ([[ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467 PubMed]
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>20735481 </pubmed>
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| ==Original publications==
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| <pubmed>1906467,18957862,20817675 24115457 </pubmed>
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| [[Category:Protein-coding genes]]
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