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| * '''Description:''' long chain acyl-CoA synthetase, involved in surfactin production <br/><br/>
| | {{#bank: LcfA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''lcfA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || long chain acyl-CoA synthetase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28560 lcfA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=lcfA lcfA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 6.119
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1680 bp, 560 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fadR]]'', ''[[yshE]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28560 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:lcfA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lcfA_2918646_2920328_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:lcfA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28560]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of lipids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[FadR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU28560
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lcfA-ysiAB-etfBA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11946]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis {{PubMed|20797616}}
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| * '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[LcfB]], [[YhfT]], [[YngI]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94547 P94547]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28560]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.3 6.2.1.3]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[lcfA]]-[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lcfA_2918646_2920328_-1 lcfA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|21398533}}
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| * '''Regulation:'''
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| ** subject to carbon catabolite repression ([[CcpA]]-[[PtsH|HPr(Ser-P)]] {{PubMed|21398533}}
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| ** induced by long chain acyl-CoA (C14 ... C20) ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression {{PubMed|21398533}}
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| ** [[FadR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}}
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| ** 1A852 ( ''lcfA''::''cat''), {{PubMed|17085570}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21398533}}
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| <pubmed>17189250,17919287 20797616 17085570 22900538 </pubmed>
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| [[Category:Protein-coding genes]]
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