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| * '''Description:''' bifunctional signal peptidase I that controls surface-adhered [[biofilm formation]] and processes [[TasA]] and [[TapA]]<br/><br/>
| | {{#bank: SipW}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sipW''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqhE ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || signal peptidase I
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24630 sipW]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SipW SipW]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sipW sipW]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.494
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 570 bp, 190 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tasA]]'', ''[[tapA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sipW_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sipW_2553930_2554502_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sipW_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24630]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein secretion]]}},
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| {{SubtiWiki category|[[biofilm formation]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[RemA regulon]]}},
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| {{SubtiWiki regulon|[[SinR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24630
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqxM-sipW-tasA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11696]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** important for [[biofilm formation]] on a solid surface, but not required at an air-liquid interface {{PubMed|22328672}}
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| ** Cleavage of hydrophobic, N-terminal signal or leader sequences from [[TasA]] and [[TapA]] {{PubMed|10049401,10559173}}
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| * '''Protein family:''' peptidase S26B family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SipW]]-[[TapA]] {{PubMed|10559173}}
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| ** [[SipW]]-[[TasA]] {{PubMed|10049401}}
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| * '''[[Localization]]:'''
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| ** membrane {{PubMed|22328672}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54506 P54506]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[tapA]]-[[sipW]]-[[tasA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sipW_2553930_2554502_-1 sipW] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
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| * '''Regulation:'''
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| ** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16430695 PubMed]
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| ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
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| ** [[SinR]]: transcription repression {{PubMed|16430695}}
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| ** [[RemA]]: transcription activation {{PubMed|23646920}}
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| * '''Additional information:'''
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| ** induction by sequestration of [[SinR]] by [[SinI]] or [[SlrA]] {{PubMed|15661000,19788541}} or by [[SlrR]] {{PubMed|20351052}}
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| ** the ''[[tapA]]-[[sipW]]-[[tasA]]'' operon is not expressed in a ''[[ymdB]]'' mutant {{PubMed|21856853}}
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| ** the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the ''[[sinR]]'' mRNA) {{PubMed|21815947}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A937 ( ''sipW''::''kan''), {{PubMed|15101989}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A937&Search=1A937 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Beth Lazazzera]], Los Angeles, USA
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>21488983 22427624 22688815 </pubmed>
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| == Original publications ==
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| <pubmed> 22328672 18047568,15175311,16430695,10368135,9694797,18430133,18647168,10464223,17720793, 20351052 10827084 21477127 10559173 23646920 21856853 21815947</pubmed>
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| [[Category:Protein-coding genes]]
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