|
|
| Line 1: |
Line 1: |
| * '''Description:''' malate-H+/Na+-lactate antiporter <br/><br/>
| | {{#bank: MleN}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''mleN''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqkI ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || malate-H+/Na+-lactate antiporter
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23560 mleN]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mleN mleN]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 7.249
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1404 bp, 468 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mleA]]'', ''[[ansB]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23560 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:mleN_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleN_2452800_2454206_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mleN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23560]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/><br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[transporters/ other]]}},
| |
| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[AnsR regulon]]}},
| |
| {{SubtiWiki regulon|[[CcpA regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU23560
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present {{PubMed|10903309}}
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11763]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:''' nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):''' [[NhaC]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54571 P54571]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23560]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
| ** ''[[ansA]]-[[ansB]]-[[mleN]]-[[mleA]]'' {{PubMed|22383849}}
| |
| ** ''[[mleN]]-[[mleA]]'' {{PubMed|22383849}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleN_2452800_2454206_-1 mleN] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:'''
| |
| ** ''[[ansA]]'': [[SigA]] {{PubMed|1711029}}
| |
| ** ''[[mleN]]'': [[SigA]] {{PubMed|22900538}}
| |
|
| |
| * '''Regulation:'''
| |
| ** ''[[ansA]]'': expressed in the presence of asparagine ([[AnsR]]) {{PubMed|11914346}}
| |
| ** ''[[mleN]]'': repressed by glucose ([[CcpA]]) {{PubMed|22900538}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[AnsR]]: transcription repression {{PubMed|11914346}}
| |
| ** [[CcpA]]: transcription repression {{PubMed|22900538}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''GP1460 (''mleN''::''spc''), available in [[Stülke]] lab
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:''' pGP388 (in [[pAC7]]), available in [[Stülke]] lab
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>18763711, 10903309 22900538 22383849 1711029 11914346</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
