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| * '''Description:''' [[response regulator aspartate phosphatase]] ([[RapA]]) inhibitor, control of the [[phosphorelay]] <br/><br/>
| | {{#bank: PhrA}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''phrA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''gsiAB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphatase ([[RapA]]) inhibitor
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12440 phrA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PhrA PhrA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=phrA phrA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 4 kDa, 10.229
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 132 bp, 44 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rapA]]'', ''[[yjpA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12440 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:phrA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phrA_1316995_1317129_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:phrA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12440]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[quorum sensing]]}},
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| {{SubtiWiki category|[[short peptides]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[ComA regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU12440
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rapA-phrA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10653]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' phr family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[RapA]]-[[PhrA]] {{PubMed|11923303,22267516}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q00829 Q00829]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU12440]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[rapA]]-[[phrA]]'' {{PubMed|1378051}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=phrA_1316995_1317129_1 phrA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|1378051}}
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| * '''Regulation:'''
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| ** repressed by glucose (4.2-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| ** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}
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| * '''Regulatory mechanism:'''
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| ** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
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| ** [[CcpA]]: transcription repression
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| ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|22267516}}
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| <pubmed>8643670,8730857,11923303,1378051,14651647,12618455, 12107147, 19380751 16091051 20238180 </pubmed>
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| [[Category:Protein-coding genes]]
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