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| * '''Description:''' glutaminase, low affinity for glutamine <br/><br/>
| | {{#bank: GlsA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''glsA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybgJ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glutaminase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || glutamine degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02430 glsA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=glsA glsA]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.315
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 981 bp, 327 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glnT]]'', ''[[glnK]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02430 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ybgJ_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glsA_264191_265174_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:glsA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02430]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[GlnL regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU02430
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ybgJ-ybgH.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12753]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' l-glutamine + H<sub>2</sub>O = L-glutamate + NH<sub>3</sub> (according to Swiss-Prot)
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| * '''Protein family:''' glutaminase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[YlaM]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3BRM 3BRM] [http://www.rcsb.org/pdb/explore.do?structureId=1MKI 1MKI]{{PubMed|18459799}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31465 O31465]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02430]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.1.2 3.5.1.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[glsA]]-[[glnT]]'' {{PubMed|15995196}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glsA_264191_265174_-1 glsA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|15995196}}
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| * '''Regulation:'''
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| ** induced in the presence of glutamine ([[GlnL]]) {{PubMed|15995196}}
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| * '''Regulatory mechanism:'''
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| ** [[GlnL]]: transcription activation {{PubMed|15995196}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>15995196,18459799 , </pubmed>
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| [[Category:Protein-coding genes]]
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