PAC5: Difference between revisions
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[[File:PAC5.jpeg|250px|thumb|right|'''pAC5: click to enlarge''']] | [[File:PAC5.jpeg|250px|thumb|right|'''pAC5: click to enlarge''']] | ||
[[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | [[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | ||
Test | |||
Vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | Vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
Revision as of 12:58, 28 October 2026
Test Vector for the construction of translational lacZ fusions that can be integrated at the amyE site
Does not replicate in B. subtilis, integrates into the amyE site
Constructed in the Rapoport lab
- in E. coli: ampicillin resistance
- in B. subtilis: chloramphenicol resistance
sequencing primers:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
<pubmed> 1619665 </pubmed>