Cca

• Description: tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3' end
  
  

Gene name	cca
Synonyms	papS, ypjI
Essential	yes PubMed
Product	tRNA nucleotidyltransferase
Function	tRNA modification
Gene expression levels in SubtiExpress: cca
MW, pI	45 kDa, 8.041
Gene length, protein length	1191 bp, 397 aa
Immediate neighbours	birA, bshA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU22450

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)

• Protein family: the protein is similar to the E. coli poly(A) polymerase

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1MIV (Geobacillus stearothermophilus 44% identity)

• UniProt: P42977

• KEGG entry: [2]

• E.C. number: 2.7.7.25

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: cca PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

<pubmed> 19883645 16364630 20155482 18523015 </pubmed>

Original publications

<pubmed>17179213,12526808,20360175 ,9829937, 16109934 </pubmed>

Operon and expression

<pubmed> 20308541 23894131</pubmed>