Revision as of 14:37, 16 May 2013

Description: extracellular polysaccharide synthesis

Gene name	epsH
Synonyms	yveR
Essential	no
Product	unknown
Function	biofilm formation
*Gene expression levels in SubtiExpress*: epsH
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	39 kDa, 5.788
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	epsI, epsG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, EAR riboswitch, SinR regulon

The gene

Basic information

Locus tag: BSU34300

Phenotypes of a mutant

altered cell death pattern in colonies PubMed

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: glycosyltransferase 2 family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization:

Database entries

Structure:

UniProt: P71057

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

Expression browser: epsH PubMed

Sigma factor: SigA PubMed

Regulation:
- repressed by SinR PubMed

Regulatory mechanism:
- SinR: transcription repression PubMed
- AbrB: transcription repression PubMed

Additional information:
- induction by sequestration of SinR by SinI or SlrA PubMed
- the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
- the EAR riboswitch (eps-associated RNA switch) located between epsB and epsC mediates processive antitermination and allows expression of the long eps operon PubMed

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Reviews

Original publications

The EAR RNA switch

Other original publications

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