Revision as of 14:03, 16 May 2013

Description: fructose-specific phosphotransferase system, EIIA component of the PTS

Gene name	levD
Synonyms
Essential	no
Product	fructose-specific phosphotransferase system, EIIA component
Function	fructose uptake and phosphorylation
*Gene expression levels in SubtiExpress*: levD
*Interactions involving this protein in SubtInteract*: LevD
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	16 kDa, 4.479
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	levE, levR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LevR regulon, SigL regulon

The gene

Basic information

Locus tag: BSU27070

Phenotypes of a mutant

Database entries

DBTBS entry: no entry

SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine (according to Swiss-Prot)

Protein family: PTS permease, mannose permease (Man) family PubMed

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:
- LevD-HPr, LevD-LevE

Localization: cytoplasm (according to Swiss-Prot)

Database entries

Structure:

UniProt: P26379

KEGG entry: [2]

E.C. number: 2.7.1.69

Additional information

Expression and regulation

Operon: levD-levE-levF-levG-sacC PubMed

Expression browser: levD PubMed

Sigma factor: SigL PubMed

Regulation:
- subject to carbon catabolite repression (CcpA, LevR) PubMed
- induced in the presence of fructose (LevR) PubMed

Regulatory mechanism:
- CcpA: transcription repression PubMed
- LevR: transcription activation PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levD_2762395_2762835_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:levD_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levD_2762395_2762835_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:levD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27070]]
 |-
 |}