Revision as of 13:23, 16 May 2013

Description: control of chemotaxis by interacting with CheD, CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs

Gene name	cheC
Synonyms	ylxJ
Essential	no
Product	CheY-P phosphatase
Function	motility and chemotaxis
*Gene expression levels in SubtiExpress*: cheC
*Interactions involving this protein in SubtInteract*: CheC
MW, pI	22 kDa, 4.035
Gene length, protein length	627 bp, 209 aa
Immediate neighbours	cheW, cheD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

Locus tag: BSU16450

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:
- CheY-P phosphatase
- controls binding of CheD to chemoreceptors in response to the levels of CheY-P PubMed

Protein family: cheC family (according to Swiss-Prot)

Paralogous protein(s):FliY

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed

Interactions:
- McpB-CheC PubMed
- CheA-CheC PubMed
- CheC-CheD PubMed, this interaction is enhanced by CheY-P PubMed
- CheC-CheY-P

Localization:

Database entries

Structure:

UniProt: P40403

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon:
- flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

Expression browser: cheC PubMed

Sigma factor:
- flgB: SigA PubMed, SigD PubMed
- ylxF: SigD PubMed

Regulation:
- repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism:
- Spo0A: transcription repression PubMed
- CodY: transcription repression PubMed
- DegU (non-phosphorylated): transcription activation PubMed

Additional information:
- in minimal medium, CheC is present with 770 +/- 330 molecules per cell PubMed

Biological materials

Mutant:
- 1A862 (no resistance), PubMed, available at BGSC

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

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+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheC_1715344_1715973_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cheC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16450]]
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