PWH844: Difference between revisions

Revision as of 15:53, 17 March 2009

pWH844

vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Hillen lab

in E. coli: ampicillin resistance

similar plasmid: pGP570

Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed

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