Revision as of 13:11, 13 May 2013

Description: sporulation-specific penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
*Gene expression levels in SubtiExpress*: dacB
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

Locus tag: BSU23190

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan

Protein family: peptidase S11 family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization:
- cell membrane (according to Swiss-Prot)

Database entries

Structure: 3MFD (beta-lactamase superfamily domain)

UniProt: P35150

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: dacB-spmA-spmB PubMed

Expression browser: dacB PubMed

Sigma factor: SigE PubMed

Regulation:
- expressed early during sporulation in the mother cell (SigE) PubMed

Regulatory mechanism:

Additional information:

Biological materials

Mutant:
- 1A744 ( dacB::cat), PubMed, available at BGSC
- 1A745 ( dacB::cat), PubMed, available at BGSC

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || carboxypeptidase
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23190 dacB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23190 dacB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.725
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spmA]]'', ''[[ypuI]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14251&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23190 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23190 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23190 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:dacB_context.gif]]