Revision as of 12:51, 13 May 2013

Description: control of chemotaxis by interacting with CheD, CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs

Gene name	cheC
Synonyms	ylxJ
Essential	no
Product	CheY-P phosphatase
Function	motility and chemotaxis
*Gene expression levels in SubtiExpress*: cheC
*Interactions involving this protein in SubtInteract*: CheC
MW, pI	22 kDa, 4.035
Gene length, protein length	627 bp, 209 aa
Immediate neighbours	cheW, cheD
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

Locus tag: BSU16450

Phenotypes of a mutant

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:
- CheY-P phosphatase
- controls binding of CheD to chemoreceptors in response to the levels of CheY-P PubMed

Protein family: cheC family (according to Swiss-Prot)

Paralogous protein(s):FliY

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed

Interactions:
- McpB-CheC PubMed
- CheA-CheC PubMed
- CheC-CheD PubMed, this interaction is enhanced by CheY-P PubMed
- CheC-CheY-P

Localization:

Database entries

Structure:

UniProt: P40403

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon:
- flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

Expression browser: cheC PubMed

Sigma factor:
- flgB: SigA PubMed, SigD PubMed
- ylxF: SigD PubMed

Regulation:
- repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism:
- Spo0A: transcription repression PubMed
- CodY: transcription repression PubMed
- DegU (non-phosphorylated): transcription activation PubMed

Additional information:
- in minimal medium, CheC is present with 770 +/- 330 molecules per cell PubMed

Biological materials

Mutant:
- 1A862 (no resistance), PubMed, available at BGSC

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU16450 cheC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16450 cheC]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CheC CheC]
@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheW]]'', ''[[cheD]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13518&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16450 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:cheC_context.gif]]