CitG: Difference between revisions
No edit summary |
|||
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle | |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle | ||
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33040 citG] | ||
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitG CitG] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitG CitG] | ||
| Line 26: | Line 26: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxN]], [[yvzF]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxN]], [[yvzF]]'' | ||
|- | |- | ||
| | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33040 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33040 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33040 Advanced_DNA] | ||
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]] | ||
Revision as of 13:48, 13 May 2013
- Description: fumarase
| Gene name | citG |
| Synonyms | |
| Essential | no |
| Product | fumarate hydratase |
| Function | TCA cycle |
| Gene expression levels in SubtiExpress: citG | |
| Interactions involving this protein in SubtInteract: CitG | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 50 kDa, 5.475 |
| Gene length, protein length | 1386 bp, 462 aa |
| Immediate neighbours | yuxN, yvzF |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33040
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
- Protein family: Fumarase subfamily (according to Swiss-Prot)
- Paralogous protein(s): AnsB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P07343
- KEGG entry: [3]
- E.C. number: 4.2.1.2
Additional information
Expression and regulation
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP718 (spec), available in Jörg Stülke's lab
- Expression vector:
- pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- lacZ fusion:
- pGP387 (in pAC7), available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
- FLAG-tag construct:
- GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed <pubmed>2509422,3923430 ,3130545 2509423 20933603</pubmed>