Revision as of 13:44, 13 May 2013

Description: Na+/malate symporter

Gene name	maeN
Synonyms	yufR
Essential	no
Product	Na+/malate symporter
Function	malate uptake
*Gene expression levels in SubtiExpress*: maeN
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	47 kDa, 8.411
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	nupQ, yufS
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

Locus tag: BSU31580

Phenotypes of a mutant

Database entries

DBTBS entry: no entry

SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family

Paralogous protein(s): CimH

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization: cell membrane (according to Swiss-Prot)

Database entries

Structure:

UniProt: O05256

KEGG entry: [2]

E.C. number:

Additional information

Expression and regulation

Operon: maeN PubMed

Expression browser: maeN PubMed

Sigma factor:

Regulation: induced by malate (MalR)

Regulatory mechanism: MalR: transcription activation

Additional information:
- the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant:
- 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
- 1A642 ( maeN::erm), PubMed, available at BGSC
- GP1449 (erm), available in Stülke lab

Expression vector:
- expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31580 maeN]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31580 maeN]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nupQ]]'', ''[[yufS]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15147&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31580 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31580 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31580 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:maeN_context.gif]]