Revision as of 14:05, 13 May 2013

Description: L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)

Gene name	tsaC
Synonyms	ipc-29d, ywlC
Essential	no
Product	L-threonylcarbamoyl-AMP synthase
Function	tRNA modification
*Gene expression levels in SubtiExpress*: tsaC
MW, pI	36 kDa, 5.82
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ywlD, ywlB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

DnaA regulon

The gene

Basic information

Locus tag: BSU36950

Phenotypes of a mutant

Database entries

DBTBS entry: no entry

SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:
- conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
- biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed

Protein family: SUA5 family (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization:

Database entries

Structure: 1HRU (the homolog from E. coli) PubMed

UniProt: P39153

KEGG entry: [2]

E.C. number:

Additional information

Expression and regulation

Operon: tsaC PubMed

Expression browser: tsaC PubMed

Sigma factor:

Regulation:

Regulatory mechanism:
- DnaA: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU36950 tsaC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36950 tsaC]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.82
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlD]]'', ''[[ywlB]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15712&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36950 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36950 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36950 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:ywlC_context.gif]]