Revision as of 13:59, 13 May 2013

Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Gene name	tagV
Synonyms	yvhJ
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
*Gene expression levels in SubtiExpress*: tagV
MW, pI	43 kDa, 9.139
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	yvyE, tagO
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance PubMed

Categories containing this gene/protein

cell wall synthesis, membrane proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

Locus tag: BSU35520

Phenotypes of a mutant

a tagV mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

DBTBS entry: [1]

SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: LytR-Cps2a-Psr family PubMed

Paralogous protein(s): TagT, TagU

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s): Mg(2+) PubMed

Effectors of protein activity:

Interactions:

Localization:
- cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed

Database entries

Structure: 3NXH

UniProt: P96499

KEGG entry: [3]

E.C. number:

Additional information

Expression and regulation

Operon: yvyE-tagV (according to DBTBS)

Expression browser: tagV PubMed

Sigma factor:

Regulation:
- repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism:
- Spo0A: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU35520 tagV]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35520 tagV]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 9.139
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvyE]]'', ''[[tagO]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15569&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35520 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yvhJ_context.gif]]