TagU

• Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
  
  

Gene name	tagU
Synonyms	lytR
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Interactions involving this protein in SubtInteract: TagU
MW, pI	34 kDa, 9.527
Gene length, protein length	918 bp, 306 aa
Immediate neighbours	lytA, mnaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU35650

Phenotypes of a mutant

• a tagU mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LytR-Cps2a-Psr family PubMed

• Paralogous protein(s): TagT, TagV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • MreB-TagU PubMed

• Localization:
  • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed

Database entries

• Structure: 3MEJ (TagT, 36% identity, 69% similarity)

• UniProt: Q02115

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tagU PubMed

• Expression browser: tagU PubMed

• Sigma factor: SigA PubMed, SigX PubMed

• Regulation:

• Regulatory mechanism:
  • LytR: autorepression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>9636707,1357079 , 19099556 21964069 </pubmed>