CheC

• Description: CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs
  
  

Gene name	cheC
Synonyms	ylxJ
Essential	no
Product	CheY-P phosphatase
Function	motility and chemotaxis
Interactions involving this protein in SubtInteract: CheC
MW, pI	22 kDa, 4.035
Gene length, protein length	627 bp, 209 aa
Immediate neighbours	cheW, cheD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CheY phosphatase, CheD regulation

• Protein family: cheC family (according to Swiss-Prot)

• Paralogous protein(s):FliY

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed

• Interactions:
  • McpB-CheC PubMed
  • CheA-CheC PubMed
  • CheC-CheD PubMed
  • CheC-CheY-P

• Localization:

Database entries

• Structure:

• UniProt: P40403

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheC PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • in minimal medium, CheC is present with 770 +/- 330 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713, 14651647, 9657996,8157612, 15175317,17609139, 16469702, 20080618,17675386,20133180 17850253 21515776</pubmed>