Revision as of 13:50, 8 December 2010

Description: 6S RNA

Gene name	bsrA
Synonyms
Essential	no
Product	6S RNA
Function	antagonist of SigA
Gene length	190 bp
Immediate neighbours	yrvM, aspS
Gene sequence (+200bp)
Genetic context

Categories containing this gene/protein

sigma factors and their control, ncRNA

This gene is a member of the following regulons

The gene

Basic information

Locus tag:

Phenotypes of a mutant

reduced growth rate PubMed

Database entries

DBTBS entry: no entry

SubtiList entry: no entry

Structure:

Additional information

Expression and regulation

Operon: bsrA PubMed

Sigma factor: SigA PubMed

Regulation: expressed in vegetative cells PubMed

Regulatory mechanism:

Additional information: transcribed as 201 nt RNA, processed to mature 190 bp 6S RNA PubMed

Biological materials

Mutant:

Expression vector:

lacZ fusion:

Labs working on this gene/RNA

Kouji Nakamura, Tsukuba, University, Japan

Roland Hartmann, Philipps-Universität Marburg, Marburg, Germany, Homepage

Your additional remarks

References

@@ Line 1: / Line 1: @@
-* '''Description:''' 4.5 S RNA<br/><br/>
+* '''Description:''' 6S RNA<br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-
 |style="background:#ABCDEF;" align="center"|'''Gene name'''
-|''scr''
+|''bsrA''
 |-
 |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
@@ Line 10: / Line 10: @@
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no
 |-
-|style="background:#ABCDEF;" align="center"| '''Product''' || small cytoplasmic RNA (scRNA, 4.5S RNA),
+|style="background:#ABCDEF;" align="center"| '''Product''' || 6S RNA
+|-
-signal recognition particle-like (SRP) component
+|style="background:#ABCDEF;" align="center"|'''Function''' || antagonist of [[SigA]]
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || presecretory protein translocation
+|style="background:#ABCDEF;" align="center"| '''Gene length''' ||  190 bp
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yaaJ]]'', ''[[dnaX]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrvM]]'', ''[[aspS]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/scr_nucleotide.txt     Gene sequence      (+200bp)   ]'''
+|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt     Gene sequence      (+200bp)   ]'''
-|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/scr_protein.txt Protein sequence]'''
 |-
-|colspan="2" | '''Genetic context''' <br/> [[Image:scr_context.gif]]
+|colspan="2" | '''Genetic context''' <br/> [[Image:BsrA_context.jpeg]]
- <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
 |}
@@ Line 28: / Line 26: @@
 __TOC__
-<br/><br/><br/><br/><br/><br/>
+<br/><br/>
+<br/><br/><br/><br/><br/>
 = [[Categories]] containing this gene/protein =
-{{SubtiWiki category|[[protein secretion]]}},
+{{SubtiWiki category|[[sigma factors and their control]]}},
 {{SubtiWiki category|[[ncRNA]]}}
@@ Line 44: / Line 43: @@
 ===Phenotypes of a mutant ===
+reduced growth rate  [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed]
 === Database entries ===
@@ Line 49: / Line 51: @@
 * '''DBTBS entry:''' no entry
-* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG00095]
+* '''SubtiList entry:''' no entry
 * '''Structure:'''
 === Additional information===
@@ Line 57: / Line 59: @@
-=The RNA=
-=== Basic information/ Evolution ===
-* '''Catalyzed reaction/ biological activity:'''
-=== Extended information on the RNA===
-* '''Kinetic information'''
-* '''Interactions:''' [[Scr]]-[[Ffh]]
-* '''Localization'''
-=== Database entries ===
-* '''Structure''':
-=== Additional information===
 =Expression and regulation=
-* '''Operon:''' ''[[yaaJ]]-[[scr]],  [[scr]]''
+* '''Operon:''' ''bsrA''  [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed]
-* '''Sigma factor:''' [[SigA]] (both promoters)
+* '''Sigma factor:''' [[SigA]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed]
-* '''Regulation:'''
+* '''Regulation:''' expressed in vegetative cells  [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed]
 * '''Regulatory mechanism:'''
-* '''Additional information:''' transcribed as 354 nt precursor, cleaved at the 5' end by [[rnc| RNase III]] and at the 3' site by [[rnjA| RNase J1]] to yield a 275 nt molecule, final trimming to 271 nt sc RNA by [[rph| RNase PH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17576666 PubMed]
+* '''Additional information:''' transcribed as 201 nt RNA, processed to mature 190 bp 6S RNA  [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed]
 =Biological materials =
@@ Line 97: / Line 80: @@
 * '''lacZ fusion:'''
-=Labs working on this gene/protein=
-[[David Bechhofer]], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
+=Labs working on this gene/RNA=
+Kouji Nakamura, Tsukuba, University, Japan
+[[Roland Hartmann]], Philipps-Universität Marburg, Marburg, Germany, [http://www.uni-marburg.de/fb16/ipc/ag_hartmann Homepage]
 =Your additional remarks=
@@ Line 105: / Line 91: @@
 =References=
-<pubmed>9346318,2468993,17576666, </pubmed>
+<pubmed>15811922,,12177353 15811922, </pubmed>
 [[Category:small RNA]]