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| * '''Description:''' ribonucleoside-diphosphate reductase (major subunit) <br/><br/>
| | {{#bank: NrdF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''nrdF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ribonucleoside-diphosphate reductase (major subunit)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 4.66
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13623]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17390
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nrdIEF-ymaB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11405]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H<sub>2</sub>O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
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| * '''Protein family:''' ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[YosP]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' [[NrdE]]-[[NrdF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P50621 P50621]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU17390]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' distinct promoters for aerobic and anaerobic expression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed], anaerobic expression requires [[ResD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| ** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| * '''Regulatory mechanism:''' [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| ** [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Elisabeth Härtig]], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>16885274,8969495,,16885274, </pubmed>
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