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| * '''Description:''' ribose-5-phosphate isomerase <br/><br/>
| | {{#bank: YwlF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ywlF''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipc-32d ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ribose-5-phosphate isomerase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.416
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 447 bp, 149 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlG]]'', ''[[ywlE]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15709]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywlF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36920
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywlFG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10942]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' lacAB/rpiB family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Reversible Mass Action Kinetics [http://www.ncbi.nlm.nih.gov/sites/entrez/956158 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** Inhibited by 6-phosphogluconate and AMP [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| ** Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ [http://www.ncbi.nlm.nih.gov/sites/entrez/956158 PubMed]
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| ** Activated by EDTA and 2-mercaptoethanol [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39156 P39156]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU36920]
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| * '''E.C. number:''' [http://www.expasy.ch/cgi-bin/nicezyme.pl?5.3.1.6 5.3.1.6]
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| === Additional information===
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| The enzyme has probably more than one active site, but with no cooperativity described [http://www.ncbi.nlm.nih.gov/sites/entrez/7144591 PubMed]
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12823818,,12823818, 19446032 956158 7144591 956158</pubmed>
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