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* '''Description:''' para-nitrobenzyl esterase <br/><br/>
{{#bank: PnbA}}


{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pnbA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''estB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || para-nitrobenzyl esterase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || lipid degradation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.776 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1467 bp, 489 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[slr]]'', ''[[padC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15444&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:pnbA_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|}
__TOC__
<br/><br/>
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU34390
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' no entry
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11844]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:''' Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot)
* '''Protein family:''' type-B carboxylesterase/lipase family (according to Swiss-Prot)
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:''' phosphorylation on Ser-189 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''Interactions:'''
* '''Localization:'''
=== Database entries ===
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1C7J 1C7J]
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37967 P37967]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU34390]
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.1.3 3.1.1.3]  3.1.1.1]
=== Additional information===
=Expression and regulation=
* '''Operon:'''
* '''[[Sigma factor]]:'''
* '''Regulation:'''
* '''Regulatory mechanism:'''
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
<pubmed>7828905,,17218307, </pubmed>

Latest revision as of 11:35, 1 August 2014