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| * '''Description:''' malate dehydrogenase <br/><br/>
| | {{#bank: Mdh}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mdh''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''citH ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || malate dehydrogenase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.727
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 936 bp, 312 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[icd]], [[phoP]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14872]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mdh_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU29120
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citZ-icd-mdh.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11386]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' (S)-malate + NAD<sup>+</sup> = oxaloacetate + NADH (according to Swiss-Prot)
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| * '''Protein family:''' MDH type 3 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/14284712 PubMed]
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| ** Inhibited by Mg2+, Ca2+, Zn2+, Ag2+ and Hg2+ [http://www.ncbi.nlm.nih.gov/pubmed/14284712 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/922015 PubMed]
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| ** Inhibited by oxaloacetate (above 1mM) and malate (above 7,7mM) [http://www.ncbi.nlm.nih.gov/pubmed/14284712 PubMed]
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| * '''Interactions:'''
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| * '''Localization:''' cytoplasm (according to Swiss-Prot), membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1EMD 1EMD] (''E.coli'')
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P49814 P49814]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29120]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.37 1.1.1.37]
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| === Additional information===
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| The enzyme is a tetramer [http://www.ncbi.nlm.nih.gov/pubmed/14284712 PubMed]
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| =Expression and regulation=
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| * '''Operon:'''
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| **''[[citZ]]-[[icd]]-[[mdh]]''
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| **''[[icd]]-[[mdh]]''
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| * '''Sigma factor:''' [[SigA]]
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| * '''Regulation:''' ''[[citZ]]'': catabolite repression ([[CcpA]]), repression by glucose + glutamate ([[CcpC]])
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| ''[[mdh]]'': constitutive
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| * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[CcpC]]: transcription repression
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP719 (spc), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** pGP1123 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>18763711 14284712 922015 </pubmed>
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| # Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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