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| * '''Description:''' GTP-binding protein involved in initiation of sporulation <br/><br/>
| | {{#bank: Obg}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''obg''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || Yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || GTP-binding protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || may be required to stimulate activity of the phosphorelay that activates Spo0A
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.859
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[spo0B]]'', ''[[pheB]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14752]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:obg_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU27920
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0B-obg.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10337]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
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| * '''Protein family:''' GTP1/OBG family (according to Swiss-Prot) Era/Obg family
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| * '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| GDP, GTP and probably ppGpp
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| Interaction with Rsb proteins.
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1LMZ 1LMZ], [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1LNZ 1LNZ]
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P20964 P20964]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27920]
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| * '''E.C. number:'''
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| === Additional information===
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| The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.
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| =Expression and regulation=
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| * '''Operon:''' ''[[spo0B]]-[[obg]]''
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| * '''Sigma factor:''' [[SigA]]
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:''' pGP835 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| *[[Bill Haldenwang]], San Antonio, USA
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| *[[Naotake Ogasawara]], Nara, Japan
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| =Your additional remarks=
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| =References=
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| # Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in ''Bacillus subtilis''. ''Microbiology'' '''148:''' 3539-3552. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed]
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| # Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein ''Structure'' '''10:''' 1581-1592. [http://www.ncbi.nlm.nih.gov/pubmed/12429099?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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| # Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes ''J. Bacteriol.'' '''182:''' 2771–2777. [http://www.ncbi.nlm.nih.gov/pubmed/10781545?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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