|
|
| (27 intermediate revisions by 6 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' P-loop ATPase <br/><br/>
| | {{#bank: TsaE}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''ydiB''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || P-loop ATPase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || unknown
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.597
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 474 bp, 158 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiL]]'', ''[[ydiC]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12410]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:ydiB_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/>
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:'''
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| reduced growth rate [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12199]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' the corresponding ''E. coli'' protein is involved in the control of the activity of the [[Gcp]] homolog [http://www.ncbi.nlm.nih.gov/sites/entrez/19376873 PubMed]
| |
|
| |
| * '''Protein family:''' UPF0079 family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:''' K(M) for ATP: 60 myM, V(max) 10 nmol/min [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''Interactions:''' dimerizes and oligomerizes [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed], the corresponding ''E. coli'' protein interacts with the protein corresponding to [[YdiC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19376873 PubMed]
| |
|
| |
| * '''Localization:''' cytoplasm with the excepton of the nucleoid region [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05515 O05515]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU05910 BSU05910]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| ATPase activity is required for ''in vivo'' function of the protein [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
|
| |
| * '''Sigma factor:'''
| |
|
| |
| * '''Regulation:'''
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:''' pGP833 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed> 17005971, 19246765 </pubmed>
| |
|
| |
| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
| |
