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| * '''Description:''' glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme<br/><br/>
| | {{#bank: GapB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gapB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ppc''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glyceraldehyde-3-phosphate dehydrogenase 2
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| |style="background:#ABCDEF;" align="center"|'''Function''' || anabolic enzyme in gluconeogenesis
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37,3 kDa, 6.47
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1020 bp, 340 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytcD]]'', ''[[speD]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14862]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gapB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:''' 2966075 - 2967094
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gapB-speD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12592]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' D-glyceraldehyde 3-phosphate + phosphate + NAD(P)<sup>+</sup> = 3-phospho-D-glyceroyl phosphate + NAD(P)H (according to Swiss-Prot) D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
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| * '''Protein family:''' glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot) glyceraldehyde-3-phosphate dehydrogenase family
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| * '''Paralogous protein(s):''' [[GapA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** Nucleotid bindinge domain (12-13)
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| ** 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34425 O34425]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29020 BSU29020]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.2.1.59 1.2.1.59] 2 1.2.1.12]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[gapB]]-[[speD]]''
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+15720552 PubMed]
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| * '''Regulation:''' repressed (70-times) by Glc, repressor [[CcpN]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+15720552 PubMed]
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| * '''Regulatory mechanism:''' transcription repression
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
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| =Your additional remarks=
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| =References=
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| <pubmed> 16479537,10799476,10844697 ,15720552,18586936,17114254</pubmed>
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