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* '''Description:''' write here <br/><br/>
{{#bank: rasP}}
* '''Description:''' intramembrane protease, cleaves [[FtsL]], [[RsiV]] and [[RsiW]] as well as signal peptides after release  of the secreted proteins<br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yluC''
|''rasP ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rasP ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yluC''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
|style="background:#ABCDEF;" align="center"| '''Product''' || intramembrane protease
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[cell division]], and [[SigV]] and [[SigW]] activity
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16560 rasP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RasP RasP]
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.14   
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.14   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1266 bp, 422 aa  
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1266 bp, 422 aa  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ispC]]'', ''[[proS]]''
|-
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yluC_nucleotide.txt    Gene sequence     (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16560 DNA_with_flanks]
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yluC_protein.txt Protein sequence]'''
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yluC_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:yluC_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rseP_1724029_1725297_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rasP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16560]]
|-
|-
|}
|}


__TOC__
__TOC__
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/><br/><br/>
<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[cell division]]}},
{{SubtiWiki category|[[proteolysis]]}},
{{SubtiWiki category|[[sigma factors and their control]]}},
{{SubtiWiki category|[[membrane proteins]]}}
= This gene is a member of the following [[regulons]] =


<br/><br/>


=The gene=
=The gene=
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=== Basic information ===
=== Basic information ===


* '''Coordinates:'''
* '''Locus tag:''' BSU16560


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
 
* defects in competence development, [[protein secretion]] and membrane protein production {{PubMed|23155385}}
* mutants grow slower in liquid, are not competent, can’t activate [[SigW]], have [[cell division]] defects, and decreased long term survival {{PubMed|23687273}}
=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16560&redirect=T BSU16560]


* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yluC.html]
* '''DBTBS entry:''' no entry


* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13410]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13410]
Line 53: Line 73:


* '''Catalyzed reaction/ biological activity:'''  
* '''Catalyzed reaction/ biological activity:'''  
** cleaves [[FtsL]], [[RsiV]] and [[RsiW]]
** cleaves signal peptides after release  of the secreted proteins {{PubMed|21810987}}


* '''Protein family:'''
* '''Protein family:''' peptidase M50B family (according to Swiss-Prot)


* '''Paralogous protein(s):'''
* '''Paralogous protein(s):'''
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* '''Effectors of protein activity:'''
* '''Effectors of protein activity:'''


* '''Interactions:'''
* '''[[SubtInteract|Interactions]]:'''
** [[RasP]]-[[FtsL]]
** [[RasP]]-[[RsiW]]
** [[RasP]]-[[RsiV]] {{PubMed|23687273}}


* '''Localization:'''
* '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]


=== Database entries ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16560&redirect=T BSU16560]


* '''Structure:'''
* '''Structure:'''


* '''Swiss prot entry:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31754 O31754]


* '''KEGG entry:'''
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16560]


* '''E.C. number:'''
* '''E.C. number:'''
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* '''Operon:'''  
* '''Operon:'''  


* '''Sigma factor:'''  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rseP_1724029_1725297_1 rasP] {{PubMed|22383849}}
 
* '''[[Sigma factor]]:'''  


* '''Regulation:'''  
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  


* '''Additional information:'''  
* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 24 {{PubMed|24696501}}


=Biological materials =
=Biological materials =
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=Labs working on this gene/protein=
=Labs working on this gene/protein=
[[Thomas Wiegert]], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas%20Wiegert-Dateien/Thomas%20Wiegert.html Homepage]


=Your additional remarks=
=Your additional remarks=


=References=
=References=
==Reviews==
<pubmed> 20836086 23479438,22381678 22688815 19189971 </pubmed>
== Original publications ==
<pubmed>16899079,15130127,18599827,17020588,19889088 ,18763711, 20644139 21810987 23687273 23155385</pubmed>


# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
[[Category:Protein-coding genes]]

Latest revision as of 11:50, 3 May 2016

rasP

  • Description: intramembrane protease, cleaves FtsL, RsiV and RsiW as well as signal peptides after release of the secreted proteins

Gene name rasP
Synonyms yluC
Essential no
Product intramembrane protease
Function control of cell division, and SigV and SigW activity
Gene expression levels in SubtiExpress: rasP
Interactions involving this protein in SubtInteract: RasP
MW, pI 46 kDa, 5.14
Gene length, protein length 1266 bp, 422 aa
Immediate neighbours ispC, proS
Sequences Protein DNA DNA_with_flanks
Genetic context
This image was kindly provided by SubtiList
Expression at a glance   PubMed















Categories containing this gene/protein

cell division, proteolysis, sigma factors and their control, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16560

Phenotypes of a mutant

  • defects in competence development, protein secretion and membrane protein production PubMed
  • mutants grow slower in liquid, are not competent, can’t activate SigW, have cell division defects, and decreased long term survival PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • cleaves FtsL, RsiV and RsiW
    • cleaves signal peptides after release of the secreted proteins PubMed
  • Protein family: peptidase M50B family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 24 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Thomas Wiegert, University of Bayreuth, Germany Homepage

Your additional remarks

References

Reviews

<pubmed> 20836086 23479438,22381678 22688815 19189971 </pubmed>

Original publications

<pubmed>16899079,15130127,18599827,17020588,19889088 ,18763711, 20644139 21810987 23687273 23155385</pubmed>

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